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analysis
method for quantitative detection of dermatan sulfate in heparin. Selective detection of deramtan sulfate in heparin is possible based on the increased value of UV absorption at 232 nm resulting from exhaustive depolymerization of dermatan sulfate. The first method facilitates analysis in heparin with dermatan sulfate concentrations greater than 0.1 mg/ml at 232 nm, with good linearity, precision and recovery. The second method allows sensitive and accurate detection of dermatan sulfate at concentrations below 0.1 mg/ml, with good linearity, precision and recovery. The linear range of dermatan sulfate detection is between 0.01 and 0.5 mg/ml
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