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biotechnology
assay targets enzymes involved in the biosynthesis of the unusual bacterial sugar diNAcBac and the transfer of diNAcBac-phosphate to UndP. This multienzyme assay, together with the established assays for the individual enzymes, can be used to screen for inhibitors, and may be used to evaluate substrate flux along the inhibited pathway. This assay is optimized for maximum sensitivity to inhibition of PglF, PglE, PglD, and PglC by balancing the enzyme concentrations such that each is partially rate determining
synthesis
sugar capsule capsular polysaccharide A (CPSA), which coats the surface of the mammalian symbiont Bacteroides fragilis, is a key mediator of mammalian immune system development, the enzyme, coupled to a Bacteroides fragilis-encoded aminotransferase (WcfR), is used in sythetic construction system for CPSA for synthesis of the rare stereoconfiguration sugar acetamido-4-amino-6-deoxygalactopyranose in a two-step coupled reaction with WcfR, overview
Results 1 - 2 of 2