EC Number   |
Application |
Reference |
|---|
   3.5.1.119 | analysis |
development of an in vitro activity assay for Dop, based on fluorescence anisotropy measurements. The assay is simple, sensitive, and compatible with a high-throughput format for screening purposes. It can be reliably and conveniently used for detailed kinetic measurements of Dop activity and can be employed with other Dop enzymes |
-, 733084 |
| 3.5.1.119 | drug development |
the Pup-proteasome enzymes are essential for full virulence and persistence in the mammalian host. As such, the Pup-proteasome enzymes are potential targets for development of antituberculosis therapeutics. Such development requires sensitive and robust assays for measurements of enzymatic activities and the effect of examined inhibitors. An in vitro activity assay for Dop, the first enzyme in the Pup-proteasome system is described, that is ased on fluorescence anisotropy measurements. This assay is simple, sensitive, and compatible with a high-throughput format for screening purposes and can be reliably and conveniently used for detailed kinetic measurements of Dop activity |
733084 |
| 3.5.1.119 | medicine |
since Dop is necessary for persistent infection by Mycobacterium tuberculosis, its selective inhibition holds potential for tuberculosis therapy |
752466 |
| 3.5.1.119 | pharmacology |
the enzyme provides an ideal target for the development of selective chemotherapies |
-, 729998 |
