EC Number   |
Application |
Reference |
|---|
   3.1.21.4 | analysis |
genetic analysis of 24 Hungarian canine parvovirus strains collected from 2004 to 2008 revealed that all of them are type 2a strains. Due to a seemingly constant point mutation present in most of the Hungarian canine parvovirus 2a strains, a previously described MboII-based rapid identification of CPV2c strains unfortunately cannot be reliably used any more |
710678 |
| 3.1.21.4 | analysis |
measurement of restriction digest efficiency using digital cell-free protein synthesis, which enables a sensitive trace analysis of undigested DNA at the single-molecule level in a PCR-free manner. The quantitative measurements reveal a considerable variation in the digest efficiency among restriction endonucleases, from less than 70% to more than 99%. None of them shows truly complete digestion within reasonably long periods of reaction time |
-, 775340 |
| 3.1.21.4 | analysis |
method for following the digestion of DNA by restriction endonucleases in real time without the use of any extrinsic dyes or labels via linear dichroism spectroscopy |
683156 |
| 3.1.21.4 | analysis |
sequential 1-day and one-pot workflow covering in vitro protein synthesis and enzymatic assays to confirm the exact function of putative restriction-modification systems. Fluorogenic molecular beacon probes containing all the probable recognition sequences, are designed and used for the fluorogenicenzyme assay |
774526 |
| 3.1.21.4 | analysis |
the enzyme can be used in DNA-based diagnostic applications |
134258 |
| 3.1.21.4 | analysis |
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA |
-, 714119 |
| 3.1.21.4 | biotechnology |
evolvement of mutant enzymes with altered DNA cleavage specificities by application of an in vivo positive and negative selection system that applies evolutionary pressure either to favor the cleavage of a desired target sequence or to disfavor the cleavage of a nontarget sequence |
683588 |
| 3.1.21.4 | biotechnology |
generation of cleavage specificities of restriction endonucleases by swapping putative target recognition domains between the type IIB enzymes AloI, PpiI from Pseudomonas putida, and TstI from Thermus scotoductus. Individual target recognition domains recognize distinct parts of the bipartite DNA targets of these enzymes and are interchangeable. Engineering of a functional type IIB restriction endonuclease having previously undescribed DNA specificity and application in generation of type II enzymes with predetermined specificity |
-, 684004 |
| 3.1.21.4 | molecular biology |
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for future studies of large sets of restriction endonucleases and their activity |
751913 |
| 3.1.21.4 | molecular biology |
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity |
-, 751913 |
