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Results 1 - 6 of 6
EC Number
Application
Commentary
Reference
analysis
assay of synthase and hemisynthase activities of glucosamine-6-phosphate synthase by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, allowing determination of both hemisynthase and synthase parameters from a single assay kinetic experiment
analysis
development of an assay for glucosamine 6-phosphate synthase that measures the production of glucosamine 6-phosphate by either following the consumption of acetyl-CoA spectrophotometrically at 230 nm or quantifying the free thiol with 5,5'-dithio-bis(2-nitrobenzoic acid), i.e. Ellman’s reagent in a discontinuous manner. Simple assay method, which can be adapted to 96-well microtiter plate format
medicine
GFPT1 mutations in congenital myasthenic syndrome patients do not appear to compromise global N-glycosylation in muscle cells. The N-glycomes of myoblasts and myotubes derived from healthy controls, GFPT1 patients, and patients with other muscular diesease exhibit broadly similar levels of branching and relative abundances of bi-, tri-, and tetra-antennary N-glycans. Although some differences are observed in the relative abundances of some of the N-glycan constituents, these variations are modest and are not confined to the GFPT1 samples
medicine
hypoxia induces gene expression in phagocyte
medicine
mean glutamine:fructose 6-phosphate amidotransferase activity is significantly higher in diabetic compared to control subjects. Plasma levels of diabetic patients also exhibit increased lipid peroxidation and protein carbonylation. Glutamine:fructose 6-phosphate amidotransferase activity is positively correlated with glutamine:fructose 6-phosphate amidotransferase mRNA, HbA1c, insulin resistance, postprandial plasma glucose and levels of thiobarbituric acid reactive substances and protein carbonyl content
medicine
target for antifungal agents
Results 1 - 6 of 6