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biosynthesis of zeaxanthin diglucoside is obtained when the gene crtX is co-transformed into Escherichia coli containing the plasmids carrying crtE, crtB, crtI, crtY, and crtZ genes required for zeaxanthin beta-D-diglucoside biosynthesis
Escherichia coli cells that express the full six carotenoid biosynthesis genes (crtE, crtB, crtI, crtY, crtZ, and crtX) of the bacterium Pantoea ananatis synthesizes zeaxanthin 3,3'-beta-D-diglucoside and 3-beta-glucosyl-3'-beta-quinovosyl zeaxanthin. The singlet oxygen-quenching activity of zeaxanthin 3,3'-beta-D-diglucoside is superior to that of zeaxanthin
Escherichia coli strain CAR001 carries the crtEXYIB operon of Enterobacter agglomerans to produce beta-carotene. Deletion of genes encoding zeaxanthin glucosyltransferase (crtX) and lycopene beta-cyclase (crtY) from the operon leads to production of 10.5 mg lycopene/l. Further optimization results in a strain producing 3.52 g lycopene/l in fed-batch fermentation
production of rare beta-carotene-modified carotenoids possessing 2-O, 2-H or 2-glucosyl and/or 3-O, 3-H or 3-glucosyl functionalities in their beta-ionone rings using a recombinant Escherichia coli approach, involving expression of carotenoid biosynthesis genes crtE, crtB, crtI, crtY, crtZ, crtX and crtG. From the cells of the recombinant Escherichia coli, caloxanthin i.e. (beta,beta-carotene-2,3,2',3'-tetrol)-3'-beta-D-glucose, zeaxanthin i.e. (beta,beta-carotene-3,3'-diol) 3,3'-beta-D-diglucoside, and nostoxanthin i.e. (beta,beta-carotene-2,3,3'-triol) are isolated and identified. Caloxanthin 3'-beta-D-glucoside displays potent 1O2 quenching activity
Results 1 - 4 of 4