EC Number   |
Application |
Reference  |
|---|
   2.1.1.56 | analysis |
cap methylation is a principal target of the antifungal activity of sinefungin |
674701 |
| 2.1.1.56 | drug development |
cap methylation is a principal target of the antifungal activity of sinefungin |
674701 |
| 2.1.1.56 | analysis |
second Trypanosoma brucei RNA (guanine N-7) cap methyltransferase, named TbCgm1, TbCmt1 is homologous with TbCgm1, consisting of a C-terminal (guanine N-7) methyltransferase domain and an N-terminal guanylyltransferase domain, which contains signature motifs found in the nucleotidyl transferase superfamily |
677098 |
| 2.1.1.56 | analysis |
enzyme is composed of catalytic vD1 (498844) and stimulatory vD12 subunits, yeast is a powerful system for suppressor analysis of interacting poxvirus proteins, screen of amino acids in vD1-C, at which mutations restore methyltransferase function in conjunction with defective vD12 proteins, reference to the crystal structure of the microsporidian cap methyltransferase suggests that distinct functional classes of suppressors are selected |
677246 |
| 2.1.1.56 | analysis |
yeast-based system for identifying and screening inhibitors against coronavirus N7-MTase using both 96-well and 384-well microtiter plates. Sinefungin effectively suppressed N7-MTase in the yeast system. The results validate the yeast assay system for inhibitor screening yet also demonstrate the difference between cell-based and in vitro biochemical assays. The yeast system is applied to the screening of 3000 natural product extracts |
733122 |
| 2.1.1.56 | synthesis |
combination of chemical synthesis and enzymatic methylation in order to produce large amounts of RNA oligonucleotides carrying a cap-0 or cap-1. Short RNAs are synthesized on solid support by the phosphoramidite 2'-O-pivaloyloxymethyl chemistry. The cap structure is then coupled by the addition of GDP after phosphorylation of the terminal 5'-OH and activation by imidazole. GpppN-RNAs or GpppN2'-Om-RNAs are purified before the N7-methyl group is added using the human (guanine-N7)-methyl transferase to yield 7mGpppN-RNAs (cap-0) or 7mGpppN29-Om-RNAs (cap-1) |
735240 |
| 2.1.1.56 | medicine |
the enzyme is a therapeutic target in breast cancer |
757896 |
