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Results 1 - 6 of 6
EC Number
Application
Commentary
Reference
analysis
cap methylation is a principal target of the antifungal activity of sinefungin
analysis
enzyme is composed of catalytic vD1 (498–844) and stimulatory vD12 subunits, yeast is a powerful system for suppressor analysis of interacting poxvirus proteins, screen of amino acids in vD1-C, at which mutations restore methyltransferase function in conjunction with defective vD12 proteins, reference to the crystal structure of the microsporidian cap methyltransferase suggests that distinct functional classes of suppressors are selected
analysis
second Trypanosoma brucei RNA (guanine N-7) cap methyltransferase, named TbCgm1, TbCmt1 is homologous with TbCgm1, consisting of a C-terminal (guanine N-7) methyltransferase domain and an N-terminal guanylyltransferase domain, which contains signature motifs found in the nucleotidyl transferase superfamily
analysis
yeast-based system for identifying and screening inhibitors against coronavirus N7-MTase using both 96-well and 384-well microtiter plates. Sinefungin effectively suppressed N7-MTase in the yeast system. The results validate the yeast assay system for inhibitor screening yet also demonstrate the difference between cell-based and in vitro biochemical assays. The yeast system is applied to the screening of 3000 natural product extracts
drug development
cap methylation is a principal target of the antifungal activity of sinefungin
synthesis
combination of chemical synthesis and enzymatic methylation in order to produce large amounts of RNA oligonucleotides carrying a cap-0 or cap-1. Short RNAs are synthesized on solid support by the phosphoramidite 2'-O-pivaloyloxymethyl chemistry. The cap structure is then coupled by the addition of GDP after phosphorylation of the terminal 5'-OH and activation by imidazole. GpppN-RNAs or GpppN2'-Om-RNAs are purified before the N7-methyl group is added using the human (guanine-N7)-methyl transferase to yield 7mGpppN-RNAs (cap-0) or 7mGpppN29-Om-RNAs (cap-1)
Results 1 - 6 of 6