EC Number   |
Application |
Reference |
|---|
   1.14.99.53 | analysis |
development of a chitinase-coupled assay method and an assay based on consumption of ascorbate. There is no H2O2 production in the presence of colloidal chitin at 4 mg/ml |
-, 740398 |
| 1.14.99.53 | analysis |
method for quantification of C1-oxidized chitooligosaccharides (aldonic acids), and hence LPMO activity |
740448 |
| 1.14.99.53 | analysis |
method to kinetically assess the cellulolytic peroxygenase reaction using competition between well characterized reference enzymes and LPMOs for the H2O2 cosubstrate. LPMOs of both bacterial and fungal origin showed high peroxygenase efficiencies, with kcat/KmH2O2 values in the order of 100-1000 mM/s |
767576 |
| 1.14.99.53 | degradation |
presence of lytic polysaccharide monooxygenase CBP21 facilitates the degradation of chitin substrates (colloidal chitin, beta-chitin, and alpha-chitin) by Chi92 |
743999 |
| 1.14.99.53 | degradation |
under optimised conditions, AA11 introduces 35 nmol of carboxylate moieties per milligram of alpha-chitin. AA11 accepts lobster shells as substrate. The use of ethyl(hydroxyimino)cyanoacetate (Oxyma) assisted click chemistry allows the rapid modification of the surface of AA11-oxidized chitins, with a fluorescent probe, a peptide, and gold nanoparticles |
-, 766874 |
| 1.14.99.53 | synthesis |
construction of a cassette vector containing the XylS/Pm system that can easily be used for exchanging LPMO coding genes with or without signal sequences. The cassette reliably produces mature (translocated) LPMOs under controlled conditions. The signal sequence of LPMO10A from Serratia marcescens gives highest levels of recombinant protein production and translocation |
744631 |
| 1.14.99.53 | synthesis |
efficient production in Escherichia coli is achieved using PelB as the most productive signal peptide for the extracellular production of CBP21 and Aeromonas veronii B565 chitinase Chi92 |
743999 |
