Any feedback?
Please rate this page
(search_result.php)
(0/150)

BRENDA support

Refine search

Search Application

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 3 of 3
download as CSV
download all results as CSV
EC Number Application Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.14.15.8synthesis a steroid 15beta-hydroxylating whole-cell solvent tolerant biocatalyst is constructed by expressing the Bacillus megaterium steroid hydroxylase CYP106A2 in the solvent tolerant Pseudomonas putida S12. Testosterone hydroxylation is improved by a factor 16 by co-expressing Fer, a putative Fe-S protein from Bacillus subtilis. The specificity for 15beta-hydroxylation is improved by mutating threonine residue 248 of CYP106A2 into valine. These insights provide the basis for an optimized whole-cell steroid-hydroxylating biocatalyst that can be applied with an organic solvent phase 699068
Display the word mapDisplay the reaction diagram Show all sequences 1.14.15.8synthesis an overexpressing Bacillus megaterium strain produces up to 115 mg/l/h 7beta-hydroxydehydroepiandrosterone in whole-cell conversions 745699
Display the word mapDisplay the reaction diagram Show all sequences 1.14.15.8synthesis efficient approach towards the preparative scale synthesis of hydroxylated steroid derivatives. Improve CYP106A2-catalyzed steroid hydroxylation towards higher productivity and quantitative product formation. Because substrate transport into the cell limits the whole-cell biotransformation, activity can be increased sixfold by using membrane-free crude cell as biocatalyst 697322
Results 1 - 3 of 3
download as CSV
download all results as CSV