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synthesis
production of (7S,8S)-7,8-dihydroxy-(9Z,12Z)-octadecadienoic acid from linoleic acid by recombinant Escherichia coli. The optimal reaction conditions are pH 7.0, 18.6°C, 10.8% (v/v) dimethyl sulfoxide, 44.9 g/l cells, and 14.3 g/l linoleic acid, with agitation at 256 rpm. Under these conditions, recombinant cells produce 7,8-dihydroxy unsaturated fatty acids in the range of 7.0-9.8 g/l from 14.3 g/l linoleic acid, 14.3 g/l oleic acid, and plant oil hydrolysates such as waste oil and olive oil containing 14.3 g/l linoleic acid or oleic acid
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