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EC Number
Organic Solvent
Commentary
Reference
5.1.3.2
diethyldicarbonate
incubation of the recombinant His6-tagged protein with increasing concentrations of diethyldicarbonate (1.0-5.0 mM) results in a time-dependent loss of activity
714602
5.1.3.2
Glycerol
the use of glycerol as a cosolvent enhances the GalE thermostability significantly. The enzyme in 50% glycerol can retain 90% of its activity, whereas only 20% activity is observed for the identically treated control enzyme
714602
5.1.3.2
urea
denaturation by 8 M urea at pH 7.0 causes 85% loss of ist secondary structure and dissociation of its constituent molecules
649445
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