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EC Number Organic Solvent Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.7.11.8urea the folding/unfolding transitions of urea-induced denaturation is monitored with the help of circular dichroism, intrinsic fluorescence, and UV absorption spectroscopies. Analysis of transition curves obtained from different probes reveal a coincidence of denaturation curves, suggesting that folding/unfolding of FASTK follows a two-state process with the midpoint (Cm) value at 3.50 M. Urea-induced denaturation curves are further analyzed to estimate change in the Gibbs free energy in the absence of urea (DGD0) associated with the equilibrium of denaturation. To get atomistic insights into the urea-induced denaturation of FASTK, an all-atom molecular dynamics simulation for 100 ns is performed 757248
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