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Organic Solvent
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2.4.1.129
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organic solvent DMSO is a component of in vitro peptidoglycan synthesis assays making use of its ability to solubilize the lipid II substrate without denaturing enzymes. DMSO at high concentration can potentially affect protein stability and protein-protein interactions. The presence of 20% DMSO severely impairs the interaction of PBP1B with LpoB. In contrast, at lower concentration of Triton X-100 (between 0.02% and 0.08%) the activity of PBP1B can be robustly assayed and its interactions with LpoB, CpoB, TolA and FtsN are maintained. Inhibitory effect of high detergent concentration on the enzyme activity. 25% Dimethylsulfoxide (DMSO) abrogates this detergent effect
759728
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