EC Number   |
Natural Substrates |
|---|
    6.3.4.15 | ATP + biotin + apo-[acetyl-CoA carboxylase 1] |
cytoplasmic acetyl-CoA carboxylase isozyme |
| 6.3.4.15 | ATP + biotin + apo-[acetyl-CoA carboxylase 2] |
mitochondrial acetyl-CoA carboxylase isozyme |
| 6.3.4.15 | ATP + biotin + apo-[acetyl-CoA carboxylase] |
- |
| 6.3.4.15 | ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] |
- |
| 6.3.4.15 | ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] |
activation by biotinylation, HCS is the enzyme responsible for specifically attaching biotin onto the mammalian biotin domains. Biotinylation is catalysed through a two-step reaction where biotin is first activated to biotinyl-5'-AMP in an ATP-dependent manner, the biotin is then transferred onto the epsilon-amino group of a specific target lysine residue. All biotin-dependent enzymes utilise the enzyme-bound biotin group for the transfer of CO2 between metabolites |
| 6.3.4.15 | ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] |
all biotin-dependent enzymes utilise the enzyme-bound biotin group for the transfer of CO2 between metabolites |
| 6.3.4.15 | ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] |
biotin protein ligase, BPL required to complete acetyl-CoA carboxylase's capability for fatty acid biosynthesis |
| 6.3.4.15 | ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] |
BPL is an essential enzyme responsible for the activation of biotin-dependent enzymes through the covalent attachment of biotin |
| 6.3.4.15 | ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] |
overall reaction, BPL catalyses transfer of biotin to an epsilon-amino group of a specific lysine residue, which is usually the 35th amino acid from C-terminal of apoBCCP and converts it to active holoBCCP which promotes fatty acid initiation and elongation |
| 6.3.4.15 | ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] |
the holo-enzyme is a multienzyme complex, in which biotin is bound to the biotin carboxyl carrier protein, binding structure, overview. All biotin-dependent enzymes utilise the enzyme-bound biotin group for the transfer of CO2 between metabolites |
