EC Number |
Natural Substrates |
---|
4.1.99.3 | cyclobutadipyrimidine (in DNA) |
- |
4.1.99.3 | cyclobutadipyrimidine (in DNA) |
the enzyme is involved in biological photoreactivation |
4.1.99.3 | cyclobutadipyrimidine (in DNA) |
photolyases utilize near-ultraviolet blue light to specifically repair the major photoproducts of UV-induced damaged DNA. The enzyme specifically repairs CPD lesions |
4.1.99.3 | cyclobutadipyrimidine (in DNA) |
the enzyme catalyses light-driven DNA repair and photoreduction, but in contrast to class I enzymes lacks a high degree of binding discrimination between UV-damaged and intact duplex DNA |
4.1.99.3 | cyclobutadipyrimidine in DNA |
DNA repair enzyme can absorb blue/ultraviolet A light as energy and split a pyrimidine dimer induced by ultraviolet radiation. PHR1 gene encodes a functional photolyase. The PHR1 transcripts are specifically enhanced by near-ultraviolet radiation (300-400 nm) and by sunlight |
4.1.99.3 | cyclobutadipyrimidine in DNA |
the enzyme is one of the main factors determining UVB sensitivity in Oryza sativa. Cultivar Sasanishiki is resistant to the damaging effects of UVB while cultivar Norin 1 is less resistant. Amino acid position 126 is Arg in cultivar Norin 1 and Gln in cultivar Sasanishiki. The single amino acid alteration from Gln to Arg leads to a deficit of CPD photolyase activity |
4.1.99.3 | cyclobutadipyrimidine in DNA |
- |
4.1.99.3 | cyclobutadipyrimidine in DNA |
DNA repair activity |
4.1.99.3 | cyclobutadipyrimidine in DNA |
the enzyme binds to DNA containing pyrimidine dimers with high affinity and then breaks the cyclobutane ring joining the two pyrimidines of the dimer in a light-dependent reaction, 300-500 nm |
4.1.99.3 | cyclobutadipyrimidine in DNA |
light-dependent(300-600 nm) monomerization of cyclobutyl pyrimidine dimers, formed between adjacent pyrimidines on the same DNA strand, upon exposure to UV irradiation, 220-320 nm |