EC Number   |
Natural Substrates |
|---|
    4.1.3.17 | 4-carboxy-4-hydroxy-2-oxoadipate |
- |
| 4.1.3.17 | 4-hydroxy-4-methyl-2-oxoglutarate |
involved in biosynthesis of gamma-substituted glutamic acid, participates in the alpha-keto acid pathway for degradation of the meta-fission product of protocatechuate, in Pseudomonas ochraceae grown on phthalate the physiolological substrate is not hydroxymethyl glutamic acid but CHA, L-4-carboxy-4-hydroxy-2-oxoadipate, which is cleaved to pyruvate and oxaloacetate |
| 4.1.3.17 | 4-hydroxy-4-methyl-2-oxoglutarate |
- |
| 4.1.3.17 | l-4-carboxy-4-hydroxy-2-oxoadipate |
- |
| 4.1.3.17 | more |
inducible by aromatic carboxylates such as phthalate |
| 4.1.3.17 | more |
the enzyme is a class II, divalent metal ion-dependent, pyruvate aldolase that catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate and 4-carboxy-4-hydroxy-2-oxoadipate into two molecules of pyruvate in the former and a molecule of each pyruvate and oxaloacetate in the latter, cf. 4-carboxy-4-hydroxy-2-oxoadipate aldolase, EC 4.1.3. The enzyme also contains a secondary oxaloacetate decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retroaldol and decarboxylase reactions |
