EC Number   |
Natural Substrates |
|---|
   3.6.4.B7 | ATP + H2O |
- |
| 3.6.4.B7 | ATP + H2O |
DNA-dependent ATPase, D-loop formation, and strand exchange activities. The enzyme is involved in homologous recombination |
| 3.6.4.B7 | ATP + H2O |
the RadA protein is a DNA-dependent ATPase, forms a nucleoprotein filament on DNA, and catalyzes DNA pairing and strand exchange |
| 3.6.4.B7 | ATP + H2O |
the enzyme catalyses efficient D-loop formation and strand exchange at temperatures of 60-70°C, capable of promoting strand transfer through at least 1200 bp of duplex DNA |
| 3.6.4.B7 | GTP + H2O |
- |
| 3.6.4.B7 | more |
formation of SsoRadA-ssDNA complexes resulting in SsoRadA nucleoprotein filaments |
| 3.6.4.B7 | more |
The RecA/RadA-facilitated strand exchange reaction occurs in two steps: (a) the recombinases bind to ssDNA, forming a nucleoprotein complex, and (b) the nucleoprotein complex invades a homologous dsDNA, such that the invading RadA-ssDNA base pairs with the complimentary strand of the dsDNA whereas the other stranded of the DNA becomes ssDNA, forming a so-called D-loop structure. The D-loop structure is a very important intermediate in DNA repair or DNA replication processes. Formation of RadA-ssDNA filaments and stabilization, rotation mechanism of the enzyme nanobiomotor, overview |
