EC Number   |
Natural Substrates |
|---|
   3.6.1.71 | adenosine-5'-diphospho-5'-(ribonucleotide)-[DNA] + H2O |
- |
| 3.6.1.71 | adenosine-5'-diphospho-5'-[DNA] + H2O |
- |
| 3.6.1.71 | adenosine-5'-diphospho-5'-[DNA] + H2O |
substrate with single strand DNA |
| 3.6.1.71 | more |
aprataxin hydrolyses abnormal 5'-AMP DNA termini formed in abortive DNA ligations |
| 3.6.1.71 | more |
APTX-mediated DNA deadenylation activity (i.e. 5'-AMP removal) is measured in extracts of cells expressing wild-type XRCC1 or the XRCC1 phosphorylation mutant, and compared with activity in APTX-deficient and APTX-complemented human cells. APTX activity is lower in extracts from Xrcc1-/- and XRCC1 phosphorylation mutant cells compared to the robust activity in extract from wild-type XRCC1 expressing cells. And APTX-mediated DNA deadenylation activity (i.e. 5'-AMP removal) is measured in cell extracts of the XRCC1 variants, and compared with activity in patient AOA1 human fibroblasts and APTX-complemented cells |
| 3.6.1.71 | more |
aprataxin acts preferentially on adenylated nicks and double-strand breaks rather than on single-stranded DNA |
