EC Number   |
Natural Substrates |
|---|
   3.6.1.59 | 7-methylguanosine 5'-diphosphate + H2O |
- |
| 3.6.1.59 | 7-methylguanosine 5'-diphosphate + H2O |
cleavage of 7-methylguanosine 5'-diphosphate generated by Dcp1/Dcp2-mediated decapping in the 5' to 3' decay pathway |
| 3.6.1.59 | 7-methylguanosine 5'-diphosphate + H2O |
eukaryotic mRNA degradation proceeds through two main pathways, both involving mRNA cap breakdown. In the 3'-5' mRNA decay pathway, mRNA body degradation generates free m7GpppN that is hydrolyzed by DcpS generating m7GMP. In the 5'-3' pathway, the recently identified human Dcp2 decapping enzyme cleaves the cap of deadenylated mRNAs to produce m7GDP and 5'-phosphorylated mRNA |
| 3.6.1.59 | 7-methylguanosine 5'-diphosphate + H2O |
mRNA degradation occurs through distinct pathways, one primarily from the 5' end of the mRNA and the second from the 3' end. Decay from the 3' end generates the m7G5'ppp5'N cap dinucleotide, which is subsequently hydrolyzed to m7Gp and nucleotidyl 5'-diphosphate in Saccharomyces cerevisiae by a scavenger decapping activity termed Dcs1p |
| 3.6.1.59 | a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] + H2O |
the enzyme decaps RNA transcripts as long as 1400 nucleotides |
| 3.6.1.59 | m7G5'ppp5'N(3'ppp5'N)n + H2O |
cleavage of 5' end m7G-oligoribonucleotide fragments generated by 3' to 5' exonucleolytic decay |
| 3.6.1.59 | m7G5'ppp5'N(3'ppp5'N)n + H2O |
eukaryotic cells utilize DcpS, a scavenger decapping enzyme, to degrade the residual cap structure following 30-50 mRNA decay, thereby preventing the premature decapping of the capped long mRNA and misincorporation of methylated nucleotides in nucleic acids |
| 3.6.1.59 | m7G5'ppp5'N(3'ppp5'N)n + H2O |
n = 1-8 |
| 3.6.1.59 | m7G5'ppp5'N(3'ppp5'N)n + H2O |
n = 1-8, decapping is an important process in the control of eukaryotic mRNA degradation. The scavenger decapping enzyme DcpS functions to clear the cell of cap structure following decay of the RNA body by catalyzing the hydrolysis of m7GpppN to m7Gp and ppN |
| 3.6.1.59 | m7G5'ppp5'N(3'ppp5'N)n + H2O |
n = 1-8, eukaryotic mRNA degradation proceeds through two main pathways, both involving mRNA cap breakdown. In the 3'-5' mRNA decay pathway, mRNA body degradation generates free m7GpppN that is hydrolyzed by DcpS generating m7GMP. In the 5'-3' pathway, the recently identified human Dcp2 decapping enzyme cleaves the cap of deadenylated mRNAs to produce m7GDP and 5'-phosphorylated mRNA |
