EC Number   |
Natural Substrates |
|---|
   3.4.22.B60 | Boc-AGPR-7-amido-4-methylcoumarin + H2O |
- |
| 3.4.22.B60 | Boc-VLK-7-amido-4-methylcoumarin + H2O |
- |
| 3.4.22.B60 | Boc-VPR-7-amido-4-methylcoumarin + H2O |
- |
| 3.4.22.B60 | Collagen type I + H2O |
cathepsin L2 cleaves collagen type I at 43 sites within the alpha1 chain and 26 sites within the alpha2 chain |
| 3.4.22.B60 | collagen type II + H2O |
cathepsin L2 exhibits collagenase activity by cleaving at multiple sites within the alpha1 and alpha2 triple helix regions (Col domains) |
| 3.4.22.B60 | more |
cathepsin L2 readily cleaves substrates with Pro in the P2 position and peptide substrates mimicking the repeating Gly-Pro-Xaa motifs |
| 3.4.22.B60 | more |
the cathepsin L zymogen is autocatalytically activated and processed to mature enzyme by incubation for 2 h at 37°C in 0.1 M sodium citrate buffer (pH 5.0) containing 2 mM dithiothreitol and 2.5 mM EDTA |
| 3.4.22.B60 | tosyl-GPK-7-amido-4-methylcoumarin + H2O |
- |
| 3.4.22.B60 | tosyl-GPR-7-amido-4-methylcoumarin + H2O |
- |
| 3.4.22.B60 | Z-Gly-Pro-Gly-Gly-Pro-Ala + H2O |
- |
