EC Number   |
Natural Substrates |
|---|
   3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O |
- |
| 3.2.1.97 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O |
- |
| 3.2.1.97 | more |
highly induced by porcine gastric mucin |
| 3.2.1.97 | more |
enzyme production is induced by mucin, the induction is inhibited by glucose and other easily assimilable carbon sources, as well as by complex nitrogen sources, addition of palmitate, lambda-carrageenan or crude mucin to a mucin-based production medium enhances enzyme production |
| 3.2.1.97 | more |
induced when asialofetuin is the sole carbon source |
| 3.2.1.97 | more |
the expression of the gene is highly induced in the presence of mucin |
| 3.2.1.97 | more |
EngBF preferably releases Galbeta1-3GalNAc from the core 1-type O-glycan, i.e. Thomsen-Friedenreich antigen or T-antigen, of mucin glycoproteins. EngBF also shows transglycosylation activity of the released disaccharide to other mono- and disaccharides |
| 3.2.1.97 | more |
SpGH101 specifically removes an O-linked disaccharide Gal-beta-1,3-GalNAc-alpha from glycoproteins |
| 3.2.1.97 | more |
the enzyme hydrolyses the O-glycosidic bonds in mucin-type O-glycan between alpha-GalNAc and Ser/Thr. EngBF is highly specific for the core 1-type O-glycan to release the disaccharide Galbeta1-3GalNAc |
| 3.2.1.97 | more |
the enzyme is responsible for catalyzing the liberation of galactosyl beta-1,3-N-acetyl-D-galactosamine linked to serine or threonine residues of mucin-type glycoproteins |
