EC Number   |
Natural Substrates |
|---|
   3.1.3.32 | 3'-phopsho-5'-hydroxy-DNA |
the enzyme is capable of converting a 3'-phosphate/5'-OH strand of nicked DNA, which is unreparable by DNA ligase into a reparable 3'-OH/5'-phosphate nick in double stranded DNA |
| 3.1.3.32 | 3'-phopsho-5'-hydroxy-DNA |
role in repair of DNA strand breaks caused by oxidative damage |
| 3.1.3.32 | 3'-phospho-5'-hydroxy-DNA + H2O |
- |
| 3.1.3.32 | 3'-phospho-5'-hydroxy-RNA + H2O |
- |
| 3.1.3.32 | 3'-phosphopolynucleotide + H2O |
- |
| 3.1.3.32 | a RNA 2',3'-cyclic phosphate end + H2O |
the enzyme also has RNA 2'-phosphatase activity |
| 3.1.3.32 | more |
acts in the repair of damaged DNA |
| 3.1.3.32 | more |
repair of endogenously created 3-phosphate, involved in Rad1/Rad10 salvage pathway |
| 3.1.3.32 | more |
polynucleotide kinase/phosphatase is a bifunctional enzyme that can phosphorylate the 5'-OH termini and dephosphorylate the 3'-phosphate termini of DNA. Recombinant GFP-tagged PfPNKP dephosphorylates single-stranded substrates or double-stranded substrates with a short 3-single-stranded overhang, but not double-stranded substrates that mimicked single-strand breaks |
| 3.1.3.32 | more |
structure of the human PNKP FHA domain bound to a doubly phosphorylated phosphopeptide derived from XRCC1, overview |
