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Natural Substrates
Commentary (Nat. Sub.)
3.1.12.1
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Cas4 is a Cas1-Cas2-dependent endonuclease that cleaves 3' overhangs of prespacers. Cas4 cleavage is sequence and site-specific and depends on the presence of a protospacer adjacent motif (PAM). Cas4 enhances prespacer processing. Mechanism and structural and mechanistic model, overview
3.1.12.1
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Cas4-1 trims and orients a duplexed DNA oligonucleotide pre-spacer. Nuclease activities of Cas4 proteins are essential to define PAM, length, and orientation of new spacers
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