EC Number   |
Natural Substrates |
|---|
  3.1.1.56 | 4-methylumbelliferyl acetate + H2O |
- |
| 3.1.1.56 | 4-methylumbelliferyl acetate + H2O |
phenotype Es-D |
| 3.1.1.56 | 4-methylumbelliferyl acetate + H2O |
esterase D is identical to sialic acid-specific O-acetylesterase, which appears to be involved in the recycling of O-acetylated sialic acid molecules |
| 3.1.1.56 | 4-methylumbelliferyl acetate + H2O |
hydrolysis via formation of covalent, negatively charged enzyme-substrate tetrahydral intermediate: nucleophilic attack of S149 on carbonyl moiety after activation by H260, intermediate stabilized in oxyanion hole by M150 and L54, H260 stabilized by D226, release of alcohol followed by nucleophilic attack of water molecule at the acidic moiety and release of acetate |
| 3.1.1.56 | more |
the enzyme catalyses the hydrolysis of S-formylglutathione, a role in formaldehyde detoxification is possible |
