EC Number   |
Natural Substrates |
|---|
   2.5.1.60 | geranylgeranyl diphosphate + Arabidopsis thaliana RAB-ATa protein |
homozygous insertion mutants (rgtb1-1 and rgtb1-2) of the major beta-subunit of Arabidopsis enzyme (RGTB1) lead to reduced enzyme activity (about 25%), 50 mM phosphate buffer, pH 7.6, 10 mM MgCl2, 5 mM DTT, 0.5 microM tritium-labelled all trans-geranylgeranyl pyrophosphate, 4 microg recombinant Arabidopsis thaliana RAB-A2a protein, 32°C, 40 min |
| 2.5.1.60 | geranylgeranyl diphosphate + protein-cysteine |
- |
| 2.5.1.60 | geranylgeranyl diphosphate + protein-cysteine |
the Rab protein Rab8-GTPase, which end with a Cys-Val-Leu-Leu motif able to serve as a substrate for either geranylgeranyl transferase I and II, but modified predominantly by either geranylgeranyl transferase II in vivo |
| 2.5.1.60 | geranylgeranyl diphosphate + protein-cysteine |
reaction is critical for membrane localization of Rab proteins and for their interaction with soluble regulatory proteins |
| 2.5.1.60 | geranylgeranyl diphosphate + protein-cysteine |
this posttranslational modification is essential for the biological activity of Rab proteins |
| 2.5.1.60 | geranylgeranyl diphosphate + protein-cysteine |
enzyme crucial for membrane association and function of Rab proteins in intracellular vesicular trafficking |
| 2.5.1.60 | geranylgeranyl diphosphate + protein-cysteine |
the enzyme catalyzes posttranslational modification of proteins, the farnesyl moieties attached to the substrates are directly involved in protein-protein interactions as well as in protein-membrane interactions |
| 2.5.1.60 | geranylgeranyl diphosphate + Rab |
His6- and gluthathione S-transferase-tagged Rab proteins such as canine Rab1a, human Rab27a, Rab5a, Rab18, Rab6a, Rab13, and mouse Rab23 |
| 2.5.1.60 | geranylgeranyl diphosphate + Rab protein |
- |
| 2.5.1.60 | geranylgeranyl diphosphate + Rab protein |
intracellular vesicular trafficking is regulated by Rab proteins, small GTPases that require posttranslational geranylgeranylation for biological activity, which is catalyzed by the enzyme, a Rab geranylgeranyl transferase in complex with the Rab escort protein, REP, overview |
