EC Number   |
Natural Substrates |
|---|
   2.4.1.10 | 2 sucrose |
- |
| 2.4.1.10 | more |
production of low molecular weight levan in permeabilized recombinant E. coli cells |
| 2.4.1.10 | more |
6-beta-D-fructosyl)n is levan |
| 2.4.1.10 | more |
in Lactobacillus sanfranciscensis TMW 1.392, sucrose metabolism and formation of fructan and 1-kestose is dependent on the activity of a single enzyme, levansucrase |
| 2.4.1.10 | more |
the product levan has not a major impact on biofilm formation |
| 2.4.1.10 | more |
thermo-responsive expression and differential secretion of the enzyme. The extracellular levansucrase is encoded by two functional genes, lscB and lscC. Transcription of lscB and lscC is temperature-dependent. Quantification of Lsc in supernatants and cellular protein samples of mutants defective in either lscB or lscC confirm that LscB secretion at low temperature is due to a combination of thermo-regulated transcription and secretion. LscC accumulates in the periplasmic space |
| 2.4.1.10 | more |
isozyme Lsc3 synthesizes a high molecular weight fructan polymer, levan, from sucrose, but it also shows formation of fructooligosaccharides with potential prebiotic effects from both sucrose and raffinose, degrees of polymerization up to five, overview. Formation of fructooligosaccharides is enhanced by increased sucrose or raffinose concentrations of 600 mM and higher. Analysis of product in underivatized form by fully automated chip-based nanoelectrospray ionization, nanoESI, high-capacity ion trap mass spectrometry, HCT-MS. Structural analysis by tandem mass spectrometry, MS/MS, employing collision-induced dissociation at low energies, overview |
| 2.4.1.10 | more |
levan, a polymer of fructose linked by fructofurano side bonds, is produced by the transfructosylation reaction of levansucrase |
| 2.4.1.10 | more |
levansucrase catalyzes the synthesis of levan from sucrose, but it may also transfer the fructosyl moiety from sucrose to acceptor molecules included in the reaction medium |
| 2.4.1.10 | more |
levan biosynthesis and fructooligosaccharide biosynthesis |
