EC Number   |
Natural Substrates |
|---|
   1.21.98.2 | 2 3-(7-chloroindol-3-yl)-2-iminopropanoate + H2O2 |
- |
| 1.21.98.2 | 4 2-imino-3-(7-chloroindol-3-yl)propanoate + O2 |
rebeccamycin biosynthetic pathway |
| 1.21.98.2 | 7-chloroindole-3-pyruvic acid + 7-chlorotryptophan + O2 |
RebD is involved in the construction of a bisindole structure by catalyzing a coupling reaction between 7-chlorotryptophan and 7-chloroindole-3-pyruvic acid |
| 1.21.98.2 | more |
RebD is a heme-containing peroxidase-like enzyme that catalyzes the oxidative linking of two tryptophan skeletons to form dichlorochromopyrrolic acid. RebD reacts as a peroxidase forming two indole-3-pyruvic acid imine radicals that recombine as a CĀC bond in the chromopyrrolic acid. When catalase is included to remove H2O2, chromopyrrolic acid can still be formed because the indole-3-pyruvic acid imine rapidly reduces RebD, which reacts with O2, utilizing oxidase-peroxidase chemistry to produce chromopyrrolic acid. Reduced RebD can also react with H2O2 forming Cpd II (state of a hemoprotein that is 1 equivalent of oxidation greater than the ferric form and contains an oxoferryl center) directly, which can oxidize indole-3-pyruvic acid imine. Competitng robust catalase activity of RebD |
