EC Number |
Natural Substrates |
---|
1.19.6.1 | 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP |
- |
1.19.6.1 | 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP |
ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin, not ferredoxin is effective in coupling electron flow to nitrogenase |
1.19.6.1 | 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP |
enzyme consists of 2 metalloproteins, Fe protein and MoFe protein, which are assumed to associate and dissociate to transfer a single electron to the substrates, termed Fe protein cycle, driven by MgATP hydrolysis, with the dissociation of the Fe protein-MoFe protein complex being the rate limiting step of the cycle |
1.19.6.1 | 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP |
anaerobic conditions are required by the enzyme |
1.19.6.1 | more |
both nifI1 and nifI2 are required for regulation in vivo |
1.19.6.1 | more |
nitrogenase catalyzes the biological reduction of N2 to ammonia as well as the two-electron reduction of the nonphysiological alkyne substrate, alkyne substrate interaction within the nitrogenase MoFe protein, overview, the addition of neither 2-butyne-1-ol nor 2-butyne-1,4-diol to the growth medium has any effect on the capacity of wild-type Azotobacter vinelandii to sustain diazotrophic growth |
1.19.6.1 | reduced ferredoxin + H+ + N2 + ATP |
- |
1.19.6.1 | reduced flavodoxin + N2 + ATP + H2O |
- |
1.19.6.1 | reduced flavodoxin II + N2 + ATP + H2O |
- |