EC Number |
Natural Substrates |
---|
1.14.14.176 | more |
cDNAs encoding taxa-4(5),11(12)-diene synthase and CYP725A4 are introduced in Nicotiana sylvestris for specific expression in trichome cells. The co-expression does not lead to the production of the expected 5-hydroxytaxa-4(20),11(12)-diene. Instead, taxa-4(5),11(12)-diene is quantitatively converted to a novel taxane |
1.14.14.176 | taxa-4(20)-11(12)-diene + AH2 + O2 |
- |
1.14.14.176 | taxa-4(5),11(12)-diene + [reduced NADPH-hemoprotein reductase] + O2 |
hydroxylation of the C-5alpha is the first of the oxidations resulting in taxol |
1.14.14.176 | taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 |
- |
1.14.14.176 | taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 |
reaction is completely dependent on NADPH, enzyme catalyzes the first oxygenating step in taxol biosynthesis pathway |
1.14.14.176 | taxadiene + [reduced NADPH-hemoprotein reductase] + O2 |
- |