EC Number   |
Natural Substrates |
|---|
    1.14.13.7 | 2 2-xylene + 2 NADPH + 2 H+ + 2 O2 |
- |
| 1.14.13.7 | 2 ethynylbenzene + NADPH + 3 O2 |
substrate only for phenol-grown cells |
| 1.14.13.7 | 3 toluene + 3 NADPH + 3 H+ + 3 O2 |
- |
| 1.14.13.7 | benzene + NADPH + O2 + H+ |
- |
| 1.14.13.7 | more |
first enzyme of phenol biodegradation |
| 1.14.13.7 | more |
phenol degradation pathway, overview |
| 1.14.13.7 | more |
only complete enzyme systems containing all three or four protein components are capable of oxidizing phenol. The electron-transfer components exert regulatory effects on substrate oxidation processes taking place at the hydroxylase actives sites, most likely through allostery. The regulatory proteins facilitate the electron-transfer step in the hydrocarbon oxidation cycle in the absence of phenol. Under these conditions, electron consumption is coupled to H2O2 formation in a hydroxylase-dependent manner |
| 1.14.13.7 | phenol + NADH + H+ + O2 |
- |
| 1.14.13.7 | phenol + NADH + H+ + O2 |
coupling between phenol hydroxylase and toluene/o-xylene monooxygenase optimizes the use of nonhydroxylated aromatic molecules by the draining effect of phenol hydroxylase on the products of oxidation catalyzed by toluene/o-xylene monooxygenase, thus avoiding phenol accumulation |
| 1.14.13.7 | phenol + NADPH + H+ + O2 |
- |
