EC Number   |
Natural Substrates |
|---|
    1.14.12.11 | 2-cresol + NADH + H+ + O2 |
activity in strain UV4 |
| 1.14.12.11 | 3-cresol + NADH + H+ + O2 |
activity in strain UV4formation of the corresponding cis-diol and catechol |
| 1.14.12.11 | 4-xylene + NADH + H+ + O2 |
activity in strain 39/D |
| 1.14.12.11 | benzene + NADH + O2 |
involved in the conversion of aromatic compounds to their corresponding catechols |
| 1.14.12.11 | ethylbenzene + NADH + O2 |
involved in the conversion of aromatic compounds to their corresponding catechols |
| 1.14.12.11 | indole + NADH + H+ + O2 |
toluene dioxygenase is ubiquitous in nature and has a broad substrate range, including benzene, toluene, ethylbenzene and xylenes. The organism produces indigo from indole through the activity of TDO |
| 1.14.12.11 | more |
the enzyme is organized in a multicomponent Rieske non-heme iron toluene 2,3-dioxygenase enzyme system. The TDO system is composed of a reductase, TDO-R, a Rieske [2Fe2S] ferredoxin, TDO-F, and a terminal dioxygenase, TDO-O, overview. TDO-F shuttles electrons from NADH via a flavin in TDO-R to TDO-O, which catalyzes the enantioselective addition of dioxygen to the aromatic nucleus to form cis-(1R,2S)-dihydroxy-3-methylcyclohexa-3,5-diene |
| 1.14.12.11 | more |
TodS exhibits basal autophosphorylation activity that increases in the presence of toluene and is translated as an increase in the rate of transphosphorylation of TodT |
| 1.14.12.11 | toluene + NADH + H+ + O2 |
- |
| 1.14.12.11 | toluene + NADH + O2 |
- |
