EC Number   |
Natural Substrates  |
|---|
   6.5.1.2 | ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
NAD+-dependent DNA ligase LigN is non-essential for cell viability. Haloferax volcanii also encodes the ATP-dependent DNA ligase LigA. As with LigN, LigA is also non-essential for cell viability. Simultaneous inactivation of both proteins is lethal, however, indicating that they share an essential function |
| 6.5.1.2 | NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
- |
| 6.5.1.2 | NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA |
| 6.5.1.2 | NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
LigA is essential for growth of Mycobacterium tuberculosis |
| 6.5.1.2 | NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
although NAD+-dependent DNA ligase is essential for mycobacterial viability, only low levels of protein are required for growth |
| 6.5.1.2 | NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
DNA ligase plays the key role in DNA replication of joining the nascent small pieces of DNA at the replication fork. The enzyme participates in the synthesis and repair of DNA |
| 6.5.1.2 | NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
the enzyme is indispensable for normal cell growth and inviability of mutants seems to be primarily the result of an inability to seal Okazaki fragments |
| 6.5.1.2 | NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
joining of short DNA fragments formed during DNA replication and so enabling DNA synthesis to progress in an overall 3'-5' direction on the antiparallel strand of the double helix, while continual 5'-3' synthesis proceeds on the other strand. Plays a role during genetic recombination and in the repair of UV-damaged DNA |
| 6.5.1.2 | NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
the enzyme plays a pivotal role in DNA replication, repair and recombination |
| 6.5.1.2 | NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m |
- |
