EC Number   |
Natural Substrates |
|---|
   3.4.21.78 | Ku70 double strand break repair protein + H2O |
- |
| 3.4.21.78 | NADH dehydrogenase (ubiquinone) Fe-S protein 3 + H2O |
- |
| 3.4.21.78 | more |
by cleaving a variety of basement membrane-associated substrates, granzyme A may disintegrate the supramolecular structure of subendothelial basement membranes in a way that allows a more rapid emigration of activated T-cells |
| 3.4.21.78 | more |
cell death-inducing serine protease |
| 3.4.21.78 | more |
compared with granzyme B, granzyme A is minor effector of target cell lysis by natural killer cells |
| 3.4.21.78 | more |
extracellular enzyme is delivered to the cytosol by perforin, where it causes a rapid increase in reactive oxygen species and mitochondrial transmembrane potential loss, but does not cleave bid or cause apoptotic factor release. Mitochondrial effect is direct, does not require cytosol, and is insensitive to bcl-2 and caspase inhibition. Mitochondrial damage is an essential first step in killer cell granule-mediated pathways of apoptosis |
| 3.4.21.78 | gasdermin B + H2O |
granzyme A from cytotoxic lymphocytes cleaves gasdermin B (GSDMB) to trigger pyroptosis in target cells, predominantly at Lys244 within the interdomain linker. This cleavage unmaskes the pore-forming activity of GSDMB. Granzyme A, delivered into GSDMB-reconstituted cells by electroporation or perforin, induces extensive pyroptosis with interdomain cleavage of GSDMB |
| 3.4.21.78 | protein NDUFS3 + H2O |
GzmA cleavage of native Ndufs3 disrupts complex I electron transport |
| 3.4.21.78 | histone H4 + H2O |
histone H4 is a substrate for GzmA in staurosporine-induced cells |
| 3.4.21.78 | more |
most abundant protease in cytotoxic T lymphocytes, granzyme A pathway of cell-mediated cytotoxicity, produced as proenzyme, activated by the dipetidyl exopeptidase cathepsin C |
