EC Number   |
Natural Substrates   |
|---|
 3.1.21.4 | DNA + H2O |
- |
 3.1.21.4 | double-stranded DNA + H2O |
- |
 3.1.21.4 | more |
BtsI recognizes and digests at GCAGTG(2/0) |
 3.1.21.4 | double-stranded DNA + H2O |
cleavage by EcoRI is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GAATTC |
 3.1.21.4 | double-stranded DNA + H2O |
cleavage by EcoRV is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GATATC |
 3.1.21.4 | double-stranded DNA + H2O |
cleavage by HindII is blunt, producing fragments with flush ends |
 3.1.21.4 | double-stranded DNA + H2O |
EcoRI recognizes 5'-GAATTC-3' while EcoRV recognizes 5'-GATATC-3', leaving overhangs and blunt DNA segments, respectively |
 3.1.21.4 | more |
EcoRV utilizes intersegmental hopping to a greater extent than does EcoRI |
 3.1.21.4 | double-stranded DNA + H2O |
LmoJ2 recognizes GCWGC (W is A or T) |
 3.1.21.4 | double-stranded DNA + H2O |
LmoJ3 recognizes GCNGC |