EC Number   |
Natural Substrates |
|---|
   3.1.21.4 | double-stranded DNA + H2O |
LmoJ2 recognizes GCWGC (W is A or T) |
| 3.1.21.4 | double-stranded DNA + H2O |
EcoRI recognizes 5'-GAATTC-3' while EcoRV recognizes 5'-GATATC-3', leaving overhangs and blunt DNA segments, respectively |
| 3.1.21.4 | double-stranded DNA + H2O |
cleavage by HindII is blunt, producing fragments with flush ends |
| 3.1.21.4 | dsDNA + H2O |
recognition sequence is 5-GGACC-3/3-CCTGG-5, enzyme cleaves between the guanosin residues at both strands |
| 3.1.21.4 | more |
the enzyme has the recognition sequence (7/13) GAYN5RTC (14/9). It excises 27 bp, and does not require S-adenosyl-L-methionine |
| 3.1.21.4 | more |
the enzyme has the recognition sequence (12/7) RCCGGY (7/12), of which it needs 2 on the substrate to be active. It excises 20 bp, and does not require S-adenosyl-L-methionine |
| 3.1.21.4 | more |
the enzyme has the recognition sequence (7/12) GAACN6CTC (13/8), of which it needs 2 on the substrate to be active. It excises 28 bp, and does not require S-adenosyl-L-methionine |
| 3.1.21.4 | more |
schematic view of the hydrogen-bond interactions of the DNA with each subunit of the protein for the 2TA and 1TA complexes, overview |
| 3.1.21.4 | more |
BtsI recognizes and digests at GCAGTG(2/0) |
| 3.1.21.4 | more |
the enzyme has the recognition sequence (10/12) GCAN6TGC (12/10), of which it needs 2 on the substrate to be active. It excises 32 bp, and requires S-adenosyl-L-methionine |
