EC Number   |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Reference |
|---|
   6.3.2.25 | -999 |
- |
two-domain structure: the two domains interact tightly under physiological conditions. The 30000 MW domain carries the binding sites for beta-tubulin and ATP. The 14000 MW domain can possibly form an additional part of the catalytic site |
1173 |
| 6.3.2.25 | 35000 |
- |
gel filtration, MW of enzyme in crude extract, the MW of the purified enzyme is 150000. Addition of pure dimeric tubulin to the 35000 MW enzyme converts it back to the larger form which is apparently a stoichiometric 1:1 complex of tubulin and the 35000 MW enzyme |
1175 |
| 6.3.2.25 | 37000 |
- |
gel filtration |
1183 |
| 6.3.2.25 | 40000 |
- |
1 * 40000, SDS-PAGE |
1166 |
| 6.3.2.25 | 40000 |
- |
glycerol density gradient centrifugation. The MW of the ligase preincubated with excess phosphocellulose-tubulin is 150000 |
1184 |
| 6.3.2.25 | 43000 |
- |
glycerol gradient centrifugation |
1166 |
| 6.3.2.25 | 43000 |
- |
x * 43000, SDS-PAGE, immunoblot |
650316 |
| 6.3.2.25 | 43425 |
- |
1 * 43425, deduced from nucleotide sequence |
653350 |
| 6.3.2.25 | 43425 |
- |
x * 43425, calculation from nucleotide sequence |
1172, 486115 |
| 6.3.2.25 | 45000 |
- |
loaded with binding partners, mass spectrometry |
709442 |
