EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Reference |
---|
3.5.1.70 | 19000 |
- |
1 * 55000 + 1 * 19000, SDS-PAGE or gel filtration in the presence of 6 M guanidine hydrochloride, two dissimilar subunits |
209226 |
3.5.1.70 | 19100 |
- |
1 * 60300 + 1 * 19100, two dissimilar subunits, calculated from nucleotide sequence |
209228 |
3.5.1.70 | 38000 |
- |
HPLC gel filtration, the large discrepancy between the molecular weight estimated by HPLC and that estimated by SDS-PAGE is due to the particularly high pI of the enzyme: the molecular weight of acylase should be 74000 Da, the sum of the values of the two subunits obtained by SDS-PAGE |
209226 |
3.5.1.70 | 55000 |
- |
1 * 55000 + 1 * 19000, SDS-PAGE or gel filtration in the presence of 6 M guanidine hydrochloride, two dissimilar subunits |
209226 |
3.5.1.70 | 60300 |
- |
1 * 60300 + 1 * 19100, two dissimilar subunits, calculated from nucleotide sequence |
209228 |
3.5.1.70 | 84070 |
- |
precursor peptide of aculeacin A acylase, calculated from nucleotide sequence |
209228 |
3.5.1.70 | 87000 |
- |
precursor peptide of aculeacin A acylase, gel filtration and SDS-PAGE |
209227 |