EC Number   |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Reference |
|---|
    3.2.1.48 | -999 |
- |
The enzyme complex is a pseudo-dimeric assembly of a correctly folded and an enzymatically active pro-SI. The sucrase subunit functions as an intramolecular chaperone implicated in the folding of isomaltase subunit. After acquisition of a correct folding in the sucrase subunit this mature form binds tightly to the isomaltase, disrupting its interaction with calnexin. The consequence is that the isomaltase subunit acquires correct folding and sucrase subunit is no longer secreted into the external milieu. |
656071 |
| 3.2.1.48 | 64000 |
- |
1 * 64000, SDS-PAGE |
655879 |
| 3.2.1.48 | 99320 |
- |
gene coding sucrase cloned in pPNG102 contains a ORF which defines a polypeptide of 99319 Da, the sequence shows similarity to previously sequenced sucrose-hydrolyzing enzymes |
136519 |
| 3.2.1.48 | 112000 |
- |
1 * 112000 + 1* 100000, hetero-dimer, 2 catalytically active sites for maltase and sucrase |
136516 |
| 3.2.1.48 | 130000 |
- |
gel filtration, density gradient, equilibrium centrifugation, sucrase subunit |
136505, 136520 |
| 3.2.1.48 | 140000 |
- |
1 * 140000 - 160000 + 1 * 140000 -160000 2 large subunits, EC 3.2.1.48 and EC 3.2.1.10, functional dimer, SDS-PAGE |
136503, 136506 |
| 3.2.1.48 | 140000 |
- |
gel filtration, luminal |
136498 |
| 3.2.1.48 | 140000 |
- |
SDS-PAGE, intact pancreatic duct, subunit I |
136508 |
| 3.2.1.48 | 140000 |
- |
SDS-PAGE, sucrase-isomaltase |
136507 |
| 3.2.1.48 | 145000 |
- |
1 * 145000, sucrase + 1* 151000, isomaltase |
136517 |
