EC Number   |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Reference |
|---|
   2.7.7.8 | -999 |
- |
PNPase, the endoribonuclease RNase E, a DEAD-RNA helicase and the glycolytic enzyme enolase are associated with a high molecular weight complex, the degradosome |
643711 |
| 2.7.7.8 | 35000 |
- |
1 * 92000 + 1 * 73000 + 1 * 35000, SDS-PAGE |
643683 |
| 2.7.7.8 | 48000 |
- |
alpha3,beta2 or alpha3,betan, x * 86000 + x * 48000, enzyme form B is obtained by keeping the ionic strength at 200 mM during purification on Sephadex G-200, at lower salt concentrations the beta subunit tends to dissociate and the enzyme reverts to the A form |
643680 |
| 2.7.7.8 | 50000 |
- |
3 * 50000, denaturing PAGE |
643689 |
| 2.7.7.8 | 51000 |
- |
4 * 51000 |
643680 |
| 2.7.7.8 | 70000 |
- |
alpha4, 4 * 70000, SDS-PAGE |
643685 |
| 2.7.7.8 | 71000 |
- |
x * 71000, enzyme form T, enzyme form I shows several bands of different molecular sizes, SDS-PAGE |
643692 |
| 2.7.7.8 | 72000 |
- |
3 * 72000, SDS-PAGE in presence of 2-mercaptoethanol |
643688 |
| 2.7.7.8 | 73000 |
- |
1 * 92000 + 1 * 73000 + 1 * 35000, SDS-PAGE |
643683 |
| 2.7.7.8 | 75000 |
- |
gel filtration |
710136 |
