EC Number   |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Reference |
|---|
   2.7.7.50 | -999 |
- |
the capping enzyme has 2 subunits: MW 95000 and 31000, the 95000 MW subunit of the vaccinia virus capping enzyme has guanylyltransferase activity, glycerol gradient centrifugation, the isolated 95000 MW guanylyltransferase can be converted to an active 60000 MW form in vitro by limited proteolysis with trypsin, the guanylyltransferase domain is localized to the amino two-thirds of the 95000 MW polypeptide |
643469 |
| 2.7.7.50 | 26400 |
- |
x * 95000, subunit containing the active site + x * 26400, transguanylyltransferase subunit, SDS-PAGE |
643466 |
| 2.7.7.50 | 28000 |
- |
catalytically active residues 211-597, i.e. RNA guanylyltransferase domain, glycerol gradient sedimentation |
643486 |
| 2.7.7.50 | 31000 |
- |
x * 95000 + x * 31000, SDS-PAGE |
643468 |
| 2.7.7.50 | 39000 |
- |
x * 45000, alpha + x * 39000, beta, SDS-PAGE, probably alpha2beta2 |
643470 |
| 2.7.7.50 | 42000 |
- |
42000 kDa fragment harbors the autoguanylylation activity |
643491 |
| 2.7.7.50 | 42000 |
- |
SDS-PAGE |
674811 |
| 2.7.7.50 | 45000 |
- |
x * 45000, alpha + x * 39000, beta, SDS-PAGE, probably alpha2beta2 |
643470 |
| 2.7.7.50 | 46000 |
- |
residues 1-210, i.e. RNA triphosphatase domain, glycerol gradient sedimentation |
643486 |
| 2.7.7.50 | 48500 |
- |
sucrose density gradient sedimentation |
643466, 643476 |
