EC Number   |
Metals/Ions |
Reference |
|---|
  6.5.1.9 | Co2+ |
42% activity compared to Mg2+ |
760446 |
| 6.5.1.9 | K+ |
875 mM used in assay conditions |
757087 |
| 6.5.1.9 | K+ |
activity strictly requires the presence of salt. Optimal activity is found at 0.5 M KC1, which can be replaced by 0.5 M K+-acetate (136%), but not by 0.5 M NaC1 (5.4%), indicating that the presence of the potassium ion is specifically needed for cDPG synthesis |
760446 |
| 6.5.1.9 | K+ |
the maximally (2.4fold) activating KC1 concentration is 0.5 M |
753559 |
| 6.5.1.9 | Mg2+ |
Km = 3.1 mM from Lineweaver-Burk plot, best cation |
760446 |
| 6.5.1.9 | Mg2+ |
required for activity |
753559 |
| 6.5.1.9 | Mn2+ |
37% activity compared to Mg2+ |
760446 |
| 6.5.1.9 | more |
Mg2+ can be partially replaced by Co2+ (42%), Mn2+ (37%), or Ni2+ (25%), but not by Ca2+, Zn2+, or Fe2+ |
760446 |
| 6.5.1.9 | Ni2+ |
25% activity compared to Mg2+ |
760446 |
