EC Number |
Metals/Ions |
Reference |
---|
6.3.2.34 | Co2+ |
divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2. Reactions containing 10 mM either MgCl2 or CoCl2 produce 40% of the F420-2 of that is produced with MnCl2 |
696202 |
6.3.2.34 | K+ |
CofE absolutely requires a monovalent cation for activity, the greatest extent of activation is achieved by K+, with maximum stimulation occurring at 0.2 M KCl, NH4+ stimulates activity to a lesser extent, extent, whereas Na+ and Li+ have no effect on CofE activity. A mixture of Mn2+, Mg2+, and K+ is the most effective |
696202 |
6.3.2.34 | Mg2+ |
divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2. Reactions containing 10 mM either MgCl2 or CoCl2 produce 40% of the F420-2 of that is produced with MnCl2. The combination of 5 mM MgCl2 and 2-5 mM of MnCl2 supports the highest activity |
696202 |
6.3.2.34 | Mn2+ |
divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2. Reactions containing 10 mM either MgCl2 or CoCl2 produce 40% of the F420-2 of that is produced with MnCl2. The combination of 5 mM MgCl2 and 2-5 mM of MnCl2 supports the highest activity |
696202 |
6.3.2.34 | Mn2+ |
highest activity in presence of both Na+ and Mn2+, assay in presence of 100 mM NaCl, 5 mM MnCl2 |
745359 |
6.3.2.34 | Mn2+ |
there are two Mn2+-binding sites per monomer within close proximity of the GDP alpha and beta-phosphate groups |
699520 |
6.3.2.34 | Na+ |
highest activity in presence of both Na+ and Mn2+, assay in presence of 100 mM NaCl, 5 mM MnCl2 |
745359 |
6.3.2.34 | NH4+ |
CofE absolutely requires a monovalent cation for activity, the greatest extent of activation is achieved by K+, NH4+ stimulates activity to a lesser extent, whereas Na+ and Li+ have no effect on CofE activity. A mixture of Mn2+, Mg2+, and K+ is the most effective |
696202 |