EC Number   |
Metals/Ions |
Reference |
|---|
  5.5.1.17 | Mg2+ |
the enzyme is mostly stimulated by 10 mM Mg2+ |
704432 |
| 5.5.1.17 | Mg2+ |
TPS6 requires a divalent metal ion, with Mg2+ and Mn2+ being effective, optimum activity with 5 mM Mg2+ and 5 mM Mn2+. Although the Km for Mn2+ is significantly lower than for Mg2+, the enzyme is more likely to operate with a Mg2+ cofactor in planta, because the concentration of Mg2+ in plant cells is about 2 orders of magnitudes higher than Mn2+. In the presence of Mn2+, the product spectrum of TPS6 is shifted toward an increased production of (S)-beta-bisabolene and a decreased production of (S)-beta-macrocarpene. KM-value for Mg2+ measured with 0.01 mM farnesyl diphosphate: 0.131 mM |
704432 |
| 5.5.1.17 | Mn2+ |
the enzyme is stimulated by 0.25 mM Mn2+. In the presence of Mn2+, there is increased formation of (S)-beta-bisabolene |
704432 |
| 5.5.1.17 | Mn2+ |
TPS6 requires a divalent metal ion, with Mg2+ and Mn2+ being effective, optimum activity with 5 mM Mg2+ and 5 mM Mn2+. Although the Km for Mn2+ is significantly lower than for Mg2+, the enzyme is more likely to operate with a Mg2+ cofactor in planta, because the concentration of Mg2+ in plant cells is about 2 orders of magnitudes higher than Mn2+. In the presence of Mn2+, the product spectrum of TPS6 is shifted toward an increased production of (S)-beta-bisabolene and a decreased production of (S)-beta-macrocarpene. KM-value for Mn2+ measured with 0.01 mM farnesyl diphosphate: 0.0234 mM |
704432 |
| 5.5.1.17 | more |
no stimulation of activity is observed in the presence of Ca2+, Cd2+, Co2+, Cu2+, Ni2+, and Zn2+ (0.25 and 5 mM each) |
704432 |
