EC Number   |
Metals/Ions |
Reference |
|---|
    5.4.2.12 | Ca2+ |
1 mm, 19% of activity with 0.5 mM Mn2+ |
650753 |
| 5.4.2.12 | Cd2+ |
divalent cation required for activity |
651716 |
| 5.4.2.12 | Co2+ |
divalent cation required for activity, 3times higher activity than with Mn2+ |
651716 |
| 5.4.2.12 | Co2+ |
required for activity, 2fold higher activity at 0.4 mM than with 0.5 mM Mn2+ |
650753 |
| 5.4.2.12 | Co2+ |
two Co2+ are coordinated within the active site |
705169 |
| 5.4.2.12 | Co2+ |
two metal sites (M1 and M2) containing cobalt ions are present in the phosphatase domain of the enzyme structure, interaction between Asp319 and the metal bound to the active site, contribution to the domain movement |
727472 |
| 5.4.2.12 | CoCl2 |
inhibition by EDTA is reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4, NiCl2, ZnCl2 |
661634 |
| 5.4.2.12 | Mg2+ |
10 mM, slight activation |
651372 |
| 5.4.2.12 | Mg2+ |
Mn2+ and Mg2+ are required for thermal stability |
679715 |
| 5.4.2.12 | Mn2+ |
absolutely required for activity, maximal activation at 1 mM |
650970 |
