EC Number   |
Metals/Ions |
Reference |
|---|
    5.1.2.1 | Iron |
one molecule of enzyme contains 2 atoms of iron |
2174 |
| 5.1.2.1 | more |
model of the assembly of the lactate racemase metallocenter, overview. Enzyme LarA receives Ni2+ from the pyridinium-3,5-bisthiocarboxylic acid mononucleotide synthase, LarE (EC 4.4.1.37) |
745850 |
| 5.1.2.1 | Ni2+ |
absolutely required. Lactobacillus plantarum possesses an organometallic nickel-containing prosthetic group. A nicotinic acid mononucleotide derivative is tethered to Lys184 and forms a tridentate pincer complex that coordinates nickel through one metal-carbon and two metal-sulfur bonds, with His200 as another ligand. Nickel-binding site structure and the role of three accessory proteins required for its activation, overview |
749373 |
| 5.1.2.1 | Ni2+ |
dependent on, nickel is an essential cofactor, the enzyme contains 19-21% nickel, measured by PAR assays and ICP-AES. The LarA Ni center is coordinated by His residues, a four coordinate square planar nickel center or a five coordinate square pyramidal site |
745850 |
| 5.1.2.1 | Ni2+ |
nickel-dependent lactate racemase |
748098 |
| 5.1.2.1 | Ni2+ |
required, the Ni-tethered pincer cofactor in enzyme LarA increases reaction barriers and destabilizes NADH-like pyruvate intermediates, due to the less electrophilic Ni cofactor and to the ring strain in the pyruvate intermediates, the Ni ion decreases the electron affinity of cofactor |
747571 |
