EC Number |
Metals/Ions |
Reference |
---|
3.5.1.25 | 1,10-phenanthroline |
results in unfolding and loss of catalytic activity at 10 mM |
754179 |
3.5.1.25 | Co2+ |
- |
246600 |
3.5.1.25 | Co2+ |
200% activation at 4 mM |
246595 |
3.5.1.25 | Co2+ |
270% increase of activity by 5 mM |
246600 |
3.5.1.25 | Co2+ |
60-150% stimulation at concentrations below 5 mM |
246589 |
3.5.1.25 | Co2+ |
restores activity after treatment with EDTA and stimulates above the control value |
246598 |
3.5.1.25 | EDTA |
results in unfolding and loss of catalytic activity at 10 mM |
754179 |
3.5.1.25 | Fe2+ |
the protein contains predominantly more zinc in a 12:1 zinc:iron ratio |
754179 |
3.5.1.25 | more |
after treatment with 10 mM 1,10-phenanthroline or 10 mM EDTA, resulting in unfolding and loss of catalytic activity, the activity cannot be restored by addition of ZnCl2 to the metal-free enzyme, indicating that the metal ions are tightly coordinated in the active site and have an essential structural role in mycobacterial NagA enzymes |
754179 |
3.5.1.25 | more |
enzyme inactivation by metal-sequestering agents and subsequent reactivation by the addition of several divalent cations |
669882 |