EC Number   |
Metals/Ions |
Reference |
|---|
   3.4.24.83 | Ca2+ |
activation ability of divalent ions decreases in the follwing order: Zn2+ Ca2+ Mn2+ Mg2+, with Mg2+ completely unable to activate the enzyme |
712372 |
| 3.4.24.83 | Co2+ |
Co2+ is capable of directly replacing lethal factors active site Zn2+ to yield a hyperactive enzyme with 2fold higher kcat value and about 2.5fold increased catalytic efficiency |
718749 |
| 3.4.24.83 | Co2+ |
Co2+ is capable to reactivate the apoprotein of lethal factor to a level comparable to that noted for the native zinc enzyme. Co2+-substituted lethal factor is not capable of killing RAW 264.7 murine macrophage-like cells |
734422 |
| 3.4.24.83 | Cu2+ |
Cu2+-substituted lethal factor, prepared by direct exchange and by apoprotein reconstitution methodologies, displays a several-fold higher catalytic competence towards chromogenic and fluorogenic lethal factor substrates than native lethal factor. Cu2+ is bound tightly with a dissociation constant in the femtomolar range. The protein-bound metal ion is coordinated to two nitrogen donor atoms, suggesting that Cu2+ binds to both active site histidine residues. Cu2+-substituted lethal factor is capable of killing RAW 264.7 murine macrophage-like cells |
734422 |
| 3.4.24.83 | Cu2+ |
replacement of Zn2+ by Cu2+ leads to an almost 30fold enhancement of the enzyme activity |
754345 |
| 3.4.24.83 | Mn2+ |
activation ability of divalent ions decreases in the follwing order: Zn2+ Ca2+ Mn2+ Mg2+, with Mg2+ completely unable to activate the enzyme |
712372 |
| 3.4.24.83 | Mn2+ |
Co2+ is capable to reactivate the apoprotein of lethal factor to a level comparable to that noted for the native zinc enzyme. Co2+-substituted lethal factor is not capable of killing RAW 264.7 murine macrophage-like cells |
734422 |
| 3.4.24.83 | more |
Mg2+ is unable to activate |
712372 |
| 3.4.24.83 | Ni2+ |
Co2+ is capable to reactivate the apoprotein of lethal factor to a level comparable to that noted for the native zinc enzyme. Co2+-substituted lethal factor is not capable of killing RAW 264.7 murine macrophage-like cells |
734422 |
| 3.4.24.83 | Zn2+ |
- |
638967 |
