EC Number |
Metals/Ions |
Reference |
---|
3.4.21.B57 | Ca2+ |
1 mM, enhances activity. An increase in CaCl2 concentration did not affect further the enzyme activity |
727459 |
3.4.21.B57 | Ca2+ |
bound at the high-affinity Ca1 site and low-affinity Ca2 site |
731311 |
3.4.21.B57 | Ca2+ |
required for enzyme stability at higher temperatures, bound Ca2+ increases the thermostability of the subtilases or protects them from autolysis |
732679 |
3.4.21.B57 | Ca2+ |
the enzyme contains seven Ca2+ ions. Four of them (Ca2-Ca5) are responsible for folding of Tk-subtilisin. Ca6 and Ca7 ions are not important for activity. The Ca1 ion is required for the maximal activity of Tk-subtilisin. Ca1, Ca6, and Ca7 ions, especially the Ca1 ion, contribute to the hyperthermostabilization of Tk-subtilisin |
726988 |
3.4.21.B57 | Ca2+ |
the enzyme requires Ca2+ for folding and assumes a molten globule-like structure in the absence of Ca2+ even in the presence of Tk-propeptide. Tk-subtilisin contains seven Ca2+-binding sites. Four of them are located within a long loop, which mostly consists of a unique insertion sequence of this protein |
707480 |
3.4.21.B57 | Ca2+ |
two Ca2+ ions bind to the beta-jelly roll domain |
728144 |
3.4.21.B57 | CaCl2 |
highest activity in the presence of 5 mM CaCl2. The enzyme exhibits 70% and 80% of the maximal activity in the presence of 1 and 100 mM CaCl2, respectively |
726706 |
3.4.21.B57 | CaCl2 |
maximum enhancement of the purified pernisine activity is observed at around 1 mM CaCl2. Further increasing the CaCl2 above 1 mM leads to a gradual decline in this enhanced activity. In the presence of 1 mM CaCl2, the proteolytic activity of pernisine is enhanced, to reach around 75% greater activity, with the maximum activity at 105.0°C |
728576 |
3.4.21.B57 | more |
NaCl and CaCl2 together do not show any cumulative effects, as they appear to separately affect the activity |
728576 |
3.4.21.B57 | NaCl |
the maximal activity of the purified pernisine seen for 20 mM NaCl in the absence of 1 mM CaCl2 increases to more than 2fold after the addition of CaCl2, with even larger relative enhancement by CaCl2 at higher NaCl concentrations |
728576 |