EC Number   |
Metals/Ions |
Reference |
|---|
   3.4.21.64 | Ca2+ |
1 mM CaCl2, the denaturation temperature of proteinase K derivatized with praseodymium (Pr) ions is 16.2°C, which is 5.9°C higher than those of metal-free and Ca2+-bound proteinase K, respectively. Isothermal titration calorimetry (ITC) measurements demonstrate that Pr-ion binding to proteinase K shows endothermic peaks, whereas Ca2+-ion binding shows exothermic peaks, indicating that the binding mode of Pr ions is different from that of Ca2+ ions, even though the crystal structures of proteinase K with Pr and Ca2+ ions are identical |
752347 |
| 3.4.21.64 | Ca2+ |
proteinase K contains two Ca2+ ions |
709583 |
| 3.4.21.64 | Ca2+ |
the native proteinase K contains two Ca2+ cations |
709581 |
| 3.4.21.64 | Calcium |
- |
29510 |
| 3.4.21.64 | Calcium |
2 calcium ions are bound to the native enzyme, activity drops by 70% if this Ca2+ is removed by EDTA |
29501 |
| 3.4.21.64 | Calcium |
required for folding of the polypeptide chain |
29501 |
| 3.4.21.64 | Calcium |
the first calcium site is formed by the loop of the residues 174-178 and Asp200 |
29501 |
| 3.4.21.64 | Calcium |
the second more mobile Ca2+ site bridges 2 loops close to the amino and the carboxy termini |
29501 |
| 3.4.21.64 | Calcium |
X-ray studies show that it has 2 binding sites for Ca2+, Ca2+ is not directly involved in the catalytic mechanism and is 16.6 A away from the alpha-carbon atoms of the catalytic triad Asp39-His69-Ser224, the activity of the enzyme towards the synthetic substrate succinyl-Ala-Ala-Ala 4-nitroanilide drops slowly to about 20% of its original value when it is depleted of Ca2+ |
29521 |
| 3.4.21.64 | Cu2+ |
proteinase K and Cu2+ ions are used to synthesize enzyme-inorganic hybrid nanoflowers (P-hNFs). The P-hNFs exhibit better activity than free proteinase K in the presence of all surfactants, i.e. CHAPS, DOC, SDS, Triton X-100 and Tergitol, except for Tween 80. synthesized enzyme-inorganic hybrid nanoflowers (P-hNFs) can potentially be used as an additive in detergent formulations |
753849 |
